Overall objectives:

1 - OBJECTIVES: To learn experimental methodologies and techniques often applied in the biomedical field SKILLS TO BE ACQUIRED BY THE STUDENT: Development of capacities at the laboratory level, including in the use of advanced scientific equipment; Development of the capacity to search and interpret the scientific information; Development of the ability to design study/experimental strategies, interpret results, and communicate those results in written and oral forms; Development of capacities for working in an autonomous way, in a team, and towards the achievement of pre-set objectives; Development of the capacity of critical thinking

Syllabus:

1 - This curricular unit comprises the development of laboratory mini-projects in the field of Biomedicine. The laboratory work will allow the student to explore various techniques such as: Culture of animal cells, including stem cells, their molecular characterization, and studies of their differentiation; Flow cytometry; Molecular Biology Methods (eg. Western Blotting, PCR, RT-PCR, etc.); ELISA techniques; Microscopy Techniques (Optical Microscopy, Electron Microscopy, Fluorescence Microscopy, Atomic Force Microscopy); Nanoparticle characterization techniques (DLS, zeta potential measurement, TRPS); UV-visible and fluorescence spectroscopy

Literature/Sources:

N/A , Artigos científicos relacionados com os miniprojectos a desenvolver ,
N/A , Scientific papers related to the mini projects to be developed ,

Assesssment methods and criteria:

Classification Type: Quantitativa (0-20)

Evaluation Methodology:
This course comprises the development of mini-projects of experimental nature. These works will be done in groups and involve several activities such as bibliographic research, programming of experimental work, execution of experiments, analysis of data and results, writing of reports, and their oral presentation/discussion. Students develop work towards a pre-defined goal by the teacher. Students' work is closely supervised by the teacher who also explains to them the fundamentals of the techniques to use. The assessment of the curricular unit has the following components: a) Student/group performance evaluation in the laboratory (continuous assessment) - 30% for the final grade; b) Written reports - 40% for the final grade; c) Oral presentations and discussion of the reports - 30% for the final grade.