Overall objectives:

1 - This curricular unit aims to introduce the fundamentals and applications of "gold standard" analytical techniques for analysis and application in the biomedical field. After completing this curricular unit, the student should be able to know the theoretical basis of separation and characterization techniques, including: chromatography (GC, LC, UHPLC and hyphenated techniques), spectroscopy (UV-Vis, FTIR), potentiometry, conductimetry, and nuclear magnetic resonance (NMR), important for the identification and characterization of biomolecules.
2 - Know the applications of each of the scientific techniques, as well as their limitations.
3 - Know how to identify the type of analysis and characterization techniques to apply to a specific research project in the field of Biomedical Engineering.
4 - Develop a critical spirit in analyzing and interpreting the results obtained in an integrated way.

Syllabus:

1 - General principles of Analytical Biochemistry. Selection of an analytical method. Validation parameters of analytical methods. Quality assurance in Analytical Biochemistry: Quality control and quality assessment.
2 - Spectroscopy. Interaction of radiation with matter. Radiation absorption in the UV-Vis and IR region. Radiation emission. Beer-Lambert Ratio; Quantitative measurements. The spectrophotometer: components. Atomic spectroscopy techniques.
3 - Separation Methods. Principles. Chromatographic techniques. GC, HPLC and UHPLC: principles and detection systems. Qualitative and quantitative analysis; Hyphenated chromatographic techniques (GC-MS and LC-MS).
4 - Electroanalytical methods. Potentiometry. pH measurement. Conductometry. Conductometric measurements. Applications. Biosensors - biological or biochemical recognition system; transducers; Electrochemical biosensors.
5 - Magnetic resonance spectroscopy (NMR). Theoretical principles and foundations. Instrumentation. The spectra. Applications.

Literature/Sources:

Manhin Basha , 2021 , Analytical Techniques in Biochemistry , Humana
Dheeraj Kumar Singh, Manik Pradhan, Arnulf Materny , 2021 , Modern Techniques of Spectroscopy: Basics, Instrumentation, and Applications , Springer
Michael W. Dong , 2019 , HPLC and UHPLC for Practicing Scientists , Wiley-VCH
Harald Günthe. , 2013 , NMR Spectroscopy: Basic Principles, Concepts and Applications in Chemistry , Wiley-VCH
Mark F. Vitha , 2018 , Spectroscopy - Principles and instrumentation , Wiley-VCH

Assesssment methods and criteria:

Classification Type: Quantitativa (0-20)

Evaluation Methodology:
In this curricular unit, 64 contact hours are planned, divided as follows: 32 theoretical hours (T), 16 theoretical-practical hours (TP), and 16 hours of laboratory practices (PL). T+TP classes will be given using audiovisual media and a whiteboard, with the teacher maintaining a permanent dialogue with the students. In PL classes, experimental work is carried out illustrating some of the themes developed in theoretical classes, the results, and conclusions of which are the subject of oral discussion. Only students who have completed the course will be admitted for T+TP assessment: mandatory attendance of all PL and delivery and discussion of the group report (1/group) PL assessment: i) continuous assessment of the laboratory; ii) Evaluation of the report and its discussion T+TP assessment: carrying out 2 theoretical tests, with a weighting of 35% each: AF=70%(T+TP)+30% PL Passing the course requires a grade >=9.5 in each of the components, T+TP and PL.