Overall objectives:

1 - Based on the knowledge already acquired in previous courses at the basic level, this course aims to empower students with knowledge (theoretical and practical) and a set of tools (experimental techniques) of great importance in biochemistry.
2 - The student is also expected to develop competencies and skills (some transversal and other specific) related to the: Capacity for teamwork; Ability for intellectual concentration and self-study; Capacity for critical thinking; Capacity for applying the acquired knowledge in new situations; Laboratory work; Ability to design experimental strategies, interpret results and communicate those results in the written form.

Syllabus:

1 - CELL CULTURE TECHNIQUES
2 - FLOW CYTOMETRY
3 - MICROSCOPY TECHNIQUES
4 - CENTRIFUGATION TECHNIQUES
5 - FLUORESCENCE SPECTROSCOPY
6 - ELECTROPHORETIC AND BLOTTING TECHNIQUES
7 - IMMUNOCHEMISTRY TECHNIQUES
8 - TECHNIQUES WITH RADIOISOTOPES

Literature/Sources:

Keith Wilson, John Walker , Principles and Techniques of Biochemistry and Molecular Biology, Cambridge University Press, 7th edition (2010) ,
R. Ian Freshney , Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Wiley-Blackwell, 7th edition (2016) ,
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr. , Biochemistry. W.H. Freeman Company, New York, 9th edition (2019) ,
Vários , Artigos de revisão ,

Assesssment methods and criteria:

Classification Type: Quantitativa (0-20)

Evaluation Methodology:
The curricular unit includes 32 hours of theoretical classes and 32 hours of practical classes. In the former, the teacher's teaching is accompanied by multimedia projection; during classes, the teacher is always open to answer questions from the students, also asking questions. The laboratory classes involve the learning/training in various laboratory techniques mentioned in the syllabus; all the experimental studies are conducted in groups. The evaluation of the UC consists of: a) 2 written tests to assess the knowledge and skills acquired by each student (60% weight); b) scientific reports of the experimental work done to assess the ability of the students to interpret the experimental results and communicate them in written form, as well as their ability to work as a team (40% weight).